Transgenic sequences are frequently lost in Phytophthora parasitica transformants without reversion of the transgene-induced silenced state.
Identifieur interne : 001D66 ( Main/Exploration ); précédent : 001D65; suivant : 001D67Transgenic sequences are frequently lost in Phytophthora parasitica transformants without reversion of the transgene-induced silenced state.
Auteurs : Elodie Gaulin [France] ; Nathalie Haget ; Moustafa Khatib ; Corentin Herbert ; Martina Rickauer ; Arnaud BottinSource :
- Canadian journal of microbiology [ 0008-4166 ] ; 2007.
Descripteurs français
- KwdFr :
- Extinction de l'expression des gènes (MeSH), Gentamicine (pharmacologie), Hygromycine (pharmacologie), Pesticides (pharmacologie), Phytophthora (effets des médicaments et des substances chimiques), Phytophthora (génétique), Protoplastes (effets des médicaments et des substances chimiques), Protéines d'algue (effets des médicaments et des substances chimiques), Protéines d'algue (génétique), Résistance aux substances (génétique), Résistance aux substances (physiologie), Spores (génétique), Transformation génétique (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Phytophthora, Protoplastes, Protéines d'algue.
- génétique : Phytophthora, Protéines d'algue, Résistance aux substances, Spores.
- pharmacologie : Gentamicine, Hygromycine, Pesticides.
- physiologie : Résistance aux substances.
- Extinction de l'expression des gènes, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Algal Proteins (drug effects), Algal Proteins (genetics), Drug Resistance (genetics), Drug Resistance (physiology), Gene Silencing (MeSH), Gentamicins (pharmacology), Hygromycin B (pharmacology), Pesticides (pharmacology), Phytophthora (drug effects), Phytophthora (genetics), Plants, Genetically Modified (MeSH), Protoplasts (drug effects), Spores (genetics), Transformation, Genetic (MeSH).
- MESH :
- chemical , drug effects : Algal Proteins.
- chemical , genetics : Algal Proteins.
- drug effects : Phytophthora, Protoplasts.
- genetics : Drug Resistance, Phytophthora, Spores.
- chemical , pharmacology : Gentamicins, Hygromycin B, Pesticides.
- physiology : Drug Resistance.
- Gene Silencing, Plants, Genetically Modified, Transformation, Genetic.
Abstract
Little data exist on the mechanism and stability of transformation in Phytophthora parasitica, a major oomycete parasite of plants. Here, we studied the stability of drug-resistant protoplast transformants by analyzing single-zoospore derivatives. We show that the transgenic sequences are not stably integrated into the chromosomes, resulting in the loss of drug resistance in single-zoospore derivatives. However, in strains where the P. parasitica gene encoding the CBEL elicitor was silenced by transformation with sense or antisense constructs, silencing is not reversed when the transgenic sequences are lost. This suggests that instability of P. parasitica transformants is not an obstacle for loss-of-function studies in this organism.
DOI: 10.1139/w06-090
PubMed: 17496962
Affiliations:
- France
- Midi-Pyrénées, Occitanie (région administrative)
- Toulouse
- Université Toulouse III - Paul Sabatier
Links toward previous steps (curation, corpus...)
Le document en format XML
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This suggests that instability of P. parasitica transformants is not an obstacle for loss-of-function studies in this organism.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17496962</PMID><DateCompleted><Year>2007</Year><Month>07</Month><Day>12</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0008-4166</ISSN><JournalIssue CitedMedium="Print"><Volume>53</Volume><Issue>1</Issue><PubDate><Year>2007</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Canadian journal of microbiology</Title><ISOAbbreviation>Can J Microbiol</ISOAbbreviation></Journal><ArticleTitle>Transgenic sequences are frequently lost in Phytophthora parasitica transformants without reversion of the transgene-induced silenced state.</ArticleTitle><Pagination><MedlinePgn>152-7</MedlinePgn></Pagination><Abstract><AbstractText>Little data exist on the mechanism and stability of transformation in Phytophthora parasitica, a major oomycete parasite of plants. Here, we studied the stability of drug-resistant protoplast transformants by analyzing single-zoospore derivatives. We show that the transgenic sequences are not stably integrated into the chromosomes, resulting in the loss of drug resistance in single-zoospore derivatives. However, in strains where the P. parasitica gene encoding the CBEL elicitor was silenced by transformation with sense or antisense constructs, silencing is not reversed when the transgenic sequences are lost. This suggests that instability of P. parasitica transformants is not an obstacle for loss-of-function studies in this organism.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gaulin</LastName><ForeName>Elodie</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>UMR5546 CNRS-Université Paul Sabatier, Castanet-Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Haget</LastName><ForeName>Nathalie</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Khatib</LastName><ForeName>Moustafa</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Herbert</LastName><ForeName>Corentin</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Rickauer</LastName><ForeName>Martina</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Bottin</LastName><ForeName>Arnaud</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Can J Microbiol</MedlineTA><NlmUniqueID>0372707</NlmUniqueID><ISSNLinking>0008-4166</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020418">Algal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005839">Gentamicins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010575">Pesticides</NameOfSubstance></Chemical><Chemical><RegistryNumber>3XQ2233B0B</RegistryNumber><NameOfSubstance UI="D006921">Hygromycin B</NameOfSubstance></Chemical><Chemical><RegistryNumber>A08F5XTI6G</RegistryNumber><NameOfSubstance UI="C010680">antibiotic G 418</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020418" MajorTopicYN="N">Algal Proteins</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004351" MajorTopicYN="N">Drug Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="N">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005839" MajorTopicYN="N">Gentamicins</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006921" MajorTopicYN="N">Hygromycin B</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010575" MajorTopicYN="N">Pesticides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="Y">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011523" MajorTopicYN="N">Protoplasts</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013170" MajorTopicYN="N">Spores</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>5</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>7</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>5</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17496962</ArticleId><ArticleId IdType="pii">w06-090</ArticleId><ArticleId IdType="doi">10.1139/w06-090</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Midi-Pyrénées</li><li>Occitanie (région administrative)</li></region><settlement><li>Toulouse</li></settlement><orgName><li>Université Toulouse III - Paul Sabatier</li></orgName></list><tree><noCountry><name sortKey="Bottin, Arnaud" sort="Bottin, Arnaud" uniqKey="Bottin A" first="Arnaud" last="Bottin">Arnaud Bottin</name><name sortKey="Haget, Nathalie" sort="Haget, Nathalie" uniqKey="Haget N" first="Nathalie" last="Haget">Nathalie Haget</name><name sortKey="Herbert, Corentin" sort="Herbert, Corentin" uniqKey="Herbert C" first="Corentin" last="Herbert">Corentin Herbert</name><name sortKey="Khatib, Moustafa" sort="Khatib, Moustafa" uniqKey="Khatib M" first="Moustafa" last="Khatib">Moustafa Khatib</name><name sortKey="Rickauer, Martina" sort="Rickauer, Martina" uniqKey="Rickauer M" first="Martina" last="Rickauer">Martina Rickauer</name></noCountry><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Gaulin, Elodie" sort="Gaulin, Elodie" uniqKey="Gaulin E" first="Elodie" last="Gaulin">Elodie Gaulin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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